To identify methods that maintain fertility when inseminating reduced numbers of valuable frozen sperm.
To use multivariate analysis to identify in-vitro tests for predicting in-vivo fertility of cryopreserved boar sperm and 2. New approaches to evaluate the fertility of frozen boar semen in vitro and methods to improve fertility when using this frozen semen with AI will allow enhanced use of superior sires though reduced sperm numbers per mating, promote single sire AI, allow sperm banking from outstanding sires, and improve the testing of semen for diseases. The use of frozen semen can help improve rates of genetic progress, improve profitability, and protect U.S. This methodology, while successful at minimizing infertility from poor quality semen, increases the risk for disease transmission and reduces the potential for genetic advancement by diluting semen from sires with superior traits. Artificial insemination (AI) is performed using 3 billion sperm in multiple inseminations using semen from multiple boars.
The pork industry relies on liquid semen with a shelf life of only five days. pork producers advantages in genetic progress and biosecurity. The current study concluded the successful usage of Tris-SBL or Tris-BHT in comparing with the possible disadvantages of using egg yolk in Tris-based extender of ram semen.Non Technical Summary We propose a research project to advance the knowledge of and practical use of frozen boar semen to provide U.S. Effects of interaction between type of extender and semen packaging method on each of TAC, MDA concentration and LDH activity were not significant. The TAC, MDA concentration and LDH activity in post-thawed semen were not affected significantly by semen packaging method. The activity of LDH was insignificantly the highest in Tris-BHT than in other extenders. TAC was higher (P<0.05), while MDA concentration was lower (P<0.05) in Tris-SBL and Tris-BHT than in Tris-EY. All sperm characteristics indicated insignificant effect of interaction between type of extender and semen packaging method. The recovery rate of motility and livability was higher at straws than in pellets. Livability and abnormality percentages were insignificantly better at straws than in pellets. Progressive motility and curled tail percentages in post-thawed semen were higher (P<0.001 P<0.05) in straws than in pellets. Results showed that sperm characteristics, including percentages of progressive motility, livability, abnormality and curled tail in post-diluted, post-equilibrated or post-thawed semen were not affected significantly by the type of extender. The concentration of total antioxidants (TAC), malondialdehyde (MDA) and lactic dehydrogenase (LDH) activity in post-thawed seminal plasma were determined. Semen was evaluated after dilution, equilibration and thawing, for progressive motility, livability and abnormality of spermatozoa and curled tail spermatozoa responded to a solution of osmolarity of 75 mOsm for 30 min. After semen extension, semen was placed for cooling in the refrigerator (5 o C) for 4 hours as equilibration period and packaged in 0.25 ml French straws or 0.25 ml pellets in liquid nitrogen. Semen was extended at a rate of 1:5 (semen/extender) with three extender types. Only semen with mass motility of ≥70% was pooled and diluted with Tris-citric extender containing 15% egg yolk (Tris-EY) or 1% soybean lecithin (Tris-SBL) or 2 mM butylated hydroxytoluene (Tris-BHT). Semen was collected from 5 sexually matured Finnish rams (50-70 kg LBW and 2-4 years old) by artificial vagina once weekly for 7 weeks. This study aimed to evaluate the effect of alternative components of egg yolk in Tris extender and semen packaging methods during cryopreservation on sperm characteristics and antioxidant system in seminal plasma of frozen-thawed semen of Finnish Landrace rams.
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